Quantitative determination of glucose-2

موضوع: Quantitative determination of glucose-2 8/4/2008, 19:58

Quantitative determination of glucose
in blood using O-toludine method

As we stated before glucose is a colorless compound dissolved in blood plasma, to be detected it must proceed a reaction with other chemical compound as O-toludine. The result of this reaction is a coloured complex (blue-green), which make the detection of glucose concentration easier.

The colour formed is directly proportional with the concentration of glucose in serum.

Deep colour high concentration of glucose.
Light colour low concentration of glucose.

Procedure:

1- Marks three test tubes as T (Test), B (Blank), S (Standard).

2- Add the following amount as shown in the table below:

Reagent	B	S	T
Glucose standard	-	0.1 ml	-
Distilled water	0.1 ml	-	-
Sample	-	-	0.1 ml
Working reagent	5ml	5ml	5ml

3- Mix well and incubate at 37 ⁰C for 8min. exactly or 30min. at RT.

“Incubation in boiling water bath”

4- Remove the tube and cool at room temp.

5- Read the A of glucose standard and test solution, using B to set spectrophotometer to Zero at wavelength (λ) = 630 nm.

Abs. (T)

Abs. (S)

X Conc. (S)

Concentration of Glucose standard: 100mg/dl

Normal range: Serum =55-110 mg/dl

موضوع: رد: Quantitative determination of glucose-2 8/4/2008, 20:08

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